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It is not derived from biological extraction but produced through controlled chemical synthesis

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For testing the difference in mean and median lifespan between groups, we used linear mixed model with linear quantile mixed-effects model, which included cage assignment as a random effect and batch as a covariate, respectively, using package nlme and lgmm in statistical software R

Li YD, Wang YB, Fan MC, Li WS, Meng XZ, Zhou H, Zhang SH, Dou QF (2023) Association of short-term nitrogen dioxide exposure with hospitalization for urolithiasis in Xinxiang, China: a time series study