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As absolute abstinence is difficult to achieve, applying hepatoprotective agents is necessary

There is also a location in Green Valley, AZ, at 514 E

8 mM of reducing agent DTT (Sigma) was then added to the samples and incubated in a thermomixer at 30C for 30 minutes, followed by the addition of 20 mM iodoacetamide (Sigma) for protein carboxylation with incubation at 30C in the dark for 30 minutes