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In mitochondria [edit] The metabolism of propionyl-CoA occurs in the mitochondria and requires Vitamin B 12 (as adenosylcobalamin) to make succinyl-CoA

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Fat Breakdown (Lipolysis) AOD-9604 promotes the breakdown of stored triglycerides into free fatty acids. This effect has been observed in adipose tissue from both lean and obese models

Elevated NNMT is linked to: Reduced NAD+ availability Slower mitochondrial function Increased fat storage efficiency Higher risk for insulin resistance By reducing NNMT activity, 5-Amino-1MQ allows the body to maintain higher NAD+ levels, which can support better mitochondrial performance, improved fat oxidation, and more balanced energy metabolism