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Researchers should match their analytical approach to the specific demands of their study, whether that means deploying HPLC-MS/MS for precise GSH/GSSG quantification, using genetically encoded biosensors like roGFP2-Grx1 for real-time subcellular redox mapping, or leveraging high-throughput fluorescent assays for large-scale screening campaigns

UPR can avoid the ERS-caused damage by reducing the accumulation of unfolded proteins and improving the correct folding of proteins, and restoring normal physiological functions of cells

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A common approach is 8-12 weeks active, 8 weeks off, then reassess whether another cycle is needed