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Subsequently, cDNA was synthesized using a poly-T Primer and the gene coding the latent jr PPO1-wt was amplified using specific primers (Table S4) and Q5 High-Fidelity DNA polymerase (NEB, Ipswich, USA)

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Across eight clinical trials involving 1,366 patients with nerve compression syndromes, PEA demonstrated both efficacy and a favorable safety profile in published literature, with no drug interactions or troublesome side effects reported