Access this article Similar content being viewed by others Abbreviations [Ca 2+ ] c : Intracellular free Ca 2+ concentration CM-H 2 DCFDA: 5-(and-6)-Chloromethyl-2,7-dichlorodihydrofluorescein diacetate, acetyl ester ER: Endoplasmic reticulum Fura-2-AM: Fura-2-acetoxymethyl ester GSH: Reduced glutathione GSSG: Oxidized glutathione HBSS: Hanks balanced salts HPSCs: Human pancreatic stellate cells H 2 O 2 : Hydrogen peroxide ROS: Reactive oxygen species RPSCs: Rat pancreatic stellate cells SERCA: Sarcoendoplasmic reticulum Ca 2+ -ATPase Tps: Thapsigargin -sma: Alpha-smooth muscle actin References Acua-Castroviejo D, Escames G, Venegas C, Daz-Casado ME, Lima-Cabello E, Lpez LC, Rosales-Corral S, Tan DX, Reiter RJ (2014) Extrapineal melatonin: sources, regulation, and potential functions
For the four-step gradient, layer 1 ml of each of 4%, 5.5%, 7% and 10% (w/v) iodixanol and layer the cell suspension on top OR for the two step gradient layer an equal volume of 9.6% (w/v) iodixanol on top of the cell suspension in 21.6% (w/v) iodixanol (see Notes 4 and 5)
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