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Surgery procedure and medication Rats were anesthetized by intraperitoneal injection of thiopental (50 mg/kg) and diazepam (5 mg/kg)

Cell 58 , 923931 (1989)

Fluorescence was recorded at 545 nm emission after 520 nm excitation for 45 minutes using a Victor3 fluorescence plate reader (PerkinElmer, USA)

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