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Protocol planning framework Step 1: Define your goals What are you hoping to achieve

The cells were cultured in Dulbeccos Modified Eagles Medium/Nutrient Mixture F-12 (DMEM/F12 = 1:1) supplemented with 5% (v/v) fetal bovine serum, 1 mM pyruvate, 50 g/mL penicillin, 50 g/mL streptomycin, 10 g/mL insulin, 5.5 g/mL transferrin, 5 ng/mL sodium selenite, and 5 ng/mL epidermal growth factor and maintained at 37 C in a sterile, humidified atmosphere of 5% CO 2

While we found no studies describing the effects of bombesins on submucosal gland activation and/or regulation of muc5B in asthma, its ability to modulate glycoprotein secretion from whole tracheas under normal physiological conditions [201] suggests a potential role for it in pathophysiological conditions

doi: 10.1007/s11306-015-0772-0 255 LaiZ-WHanczkoRBonillaECazaTNClairBBartosAet al