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The reduced activity of antioxidant enzymes in the body results in DNA hydroxylation, protein denaturation, increased lipid peroxidation, and impairment of cellular functions, disrupting the conditions necessary for cell survival

Twenty grams were blended for 2 minutes with cold chloroform-methanol mixture (2:1 v v -1 ) in proportion of 10 mL of solvent mixture per gram of tissue (except 20 ml of solvent per gram of liver tissue) in a Warring blender

The landmark 2017 Cell study (Baar MP et al., PMID: 28340339) demonstrated that: (1) FOXO4 protein specifically accumulates in senescent cells where it binds to p53 and prevents p53 from triggering apoptosis (this is the specific mechanism being targeted)

Refrain from hot showers/baths for the initial 24 hours