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Methods: A single-center, open-label trial conducted in the Philippines included 104 randomized patients to treatment (n = 52) and control (n = 52) groups

K-means clustering of amplicon sequence variant data confirmed that 5-Amino-1MQ treatment generated a distinct cecal microbiome profile from all other treatment groups including diet-switched vehicle controls indicating that NNMT inhibition produces microbiome changes that are separable from those produced by dietary composition alone