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Cell pellets were then lysed in 50 l of 5% SDS, 100 mM TEAB and sonicated (QSonica) for 5 cycles, with a 1 min resting period on ice after each cycle

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Vitamin C: Enhances collagen production, brightens the skin, and reduces discoloration

Upon its efflux from cells, oxidised glutathione is exposed to the sequential action of enzymes located on the external face of the cell membrane: -glutamyl transpeptidase (GGT) generates glutamate 94 and the cysteinylglycine dipeptide, which is degraded by dipeptidases such as aminopeptidase N (APN), thereby releasing cysteine and glycine