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This alteration stabilizes the protein and interferes with the normal activity of FOXO4

Each lysate was prewarmed in a 37 C heat block for 5 min before 103 l prewarmed (37 C) reagent mixture was added such that each final reaction had 0.1 M KPO 4 (pH 7), 0.5 mM NADPH (Cayman, 9000743), 20 nmol acetyl-CoA (Cayman, 16160), 12 mM DTT (Millipore, 3483-12-3), 20 nmol [12]C-malonyl CoA (Cayman, 16455), 12 mM EDTA and 0.1 Ci 14 C-malonyl CoA (American Radiolabeled Chemicals, ARC 0755)

JBT performed HAEC culture

In general, though, some differences in effector function can be anticipated for nAbs that have different angles of approach to their epitopes and cluster differently on the virion or infected cell surface