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[DOI] [PMC free article] [PubMed] [Google Scholar] 28.Widjaja G et al

Specifically, IR affects the expression and integrity of mtDNA-encoded genes such as 16S ribosomal RNA (16S rRNA), ATP synthase F0 subunit 6 (ATP6), and cytochrome c oxidase subunits 1, 2, and 3 (COX1, COX2, and COX3), which are essential for mitochondrial oxidative phosphorylation and energy production

Temperature cycling, repeatedly moving between refrigerator and room temperature, accelerates degradation beyond what either constant temperature would cause

In men, studies demonstrate that it can increase testosterone levels, suggestion potentially it could be used as a post cycle therapy or TRT testosterone replacement therapy