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Quantification of TfR1 expression on K1 cells was performed by determining the percentage of TfR1 + cells in each of three low magnification fields (20x)

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Interestingly, when the gdh2 / gdh2 mutant utilised glucose as the sole carbon source (MM medium), most of the intracellular metabolites were found at lower concentrations than in the wild-type strain except for cysteine, malonate, nicotinate, 9-heptadecenoate, and 2-phosphoenolpyruvate