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This is thought to occur because NADH is an inhibitor of these enzymes, so lowering it releases the inhibition.[4] Transferring electrons in NAD(P) redox reactions does not have a large effect on the total pool of NAD(P), since, rather than being broken down (catabolized), the NAD(P) molecule is interconverted between oxidized and reduced forms

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MNC were suspended in 90% fetal calf serum (FCS, Thermo Scientific, Paisley, UK) and 10% dimethyl sulfoxide (DMSO, Origen Biomedical, Solihull, UK) and stored in liquid nitrogen prior to use

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