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Optimisation of reaction conditions using 70 equivalents of vinyl palmitate, one equivalent of DPAP and DMF solvent, resulted in 91% conversion, with 97:3 ratio of mono - to bis -palmitoylated product

Begin with a low-concentration retinol (0.250.5%) a few nights per week and build up gradually

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and the content of proteins was quantified with BCA assay (Thermo Fisher Scientific, Waltham, MA, USA)