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SASP (Senescence-Associated Secretory Phenotype) signaling begins declining as the source cells are eliminated Weeks 2-6: Tissue microenvironments improve as SASP-driven inflammation decreases

QPCR was performed using the Universal Probe Library system (Roche) with the following primer/probe combinations (53), also listed in Table S1: FOXO1 (NM_002015.3): Fwd: tggttttagaaacccaagttcc, Rev: ttggcaccaagttcagttaca

People with primary insomnia show lower activity of a key antioxidant enzyme and higher signs of oxidative stress compared to healthy sleepers

While lifestyle and genetics play roles, biological factors dominate