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Samples are then rinsed 2 times in PBS then incubated in DAPI (1:3000) in PBS for 5 minutes prior to mounting on slides

Imaging of 20 days adult flies showed the neuronal architecture of Sp treated park 13 flies similar to the wild type controls whereas their untreated counterparts showed shrinkage, aggregation, and complete disruption of dopaminergic neural networks (Fig

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