In our bulk RNAseq data, we noted that pathways such as oxidative phosphorylation and electron transport chain were significantly downregulated ( Supplementary Figures 5B, C )
aureus strains LAC, HG003, MW2, JE2, JE2-lux (JE2 lux ABDCE) (kindly provided by Roger Plaut, Center for Biologics Evaluation and Research, Silver Spring) 28 , G4 (kindly provided by Anthony Richardson, University of Pittsburgh, Pittsburgh) 35 and bacteraemia isolates 8 were routinely cultured in tryptic soy broth (TSB, Fisher Scientific) or MuellerHinton broth (MHB, Oxoid) at 37 C with shaking (225 r.p.m.) unless otherwise stated
Common mistakes that sabotage results After years of accumulating user experiences and research, clear patterns emerge around what goes wrong
(2012) The down-regulation of miR-125b in chronic lymphocytic leukemias leads to metabolic adaptation of cells to a transformed state, Blood , 120 , 2631-2638, doi: Wang, F., Li, L., Chen, Z., Zhu, M., and Gu, Y