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Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18

Reporting shows that more than $83 million in federal research funding was canceled and around 383 NIH-funded clinical trials lost support within six months, exposing how vulnerable clinical timelines are to political and budgetary decisions beyond the protocol

A reservoir type system is found suitable in the present case because of following reasons

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