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Your potassium levels inside the cell are poor, your magnesium levels are poor

Kit Components Pre-coated 96-well microplate Wash Buffer Positive Control Biotinylated Detection Antibody Biotinylated Anti-Positive Control HRP-Streptavidin concentrate TMB One-Step Substrate Stop Solution Assay Diluent Sample Diluent Additional Materials Required Microplate reader capable of measuring absorbance at 450 nm Precision pipettes to deliver 2 L to 1 mL volumes Adjustable 1-25 mL pipettes for reagent preparation 100 mL and 1 liter graduated cylinders Absorbent paper Distilled or deionized water Assay Procedure Summary Prepare all reagents, samples and standards as instructed

Figure 1 demonstrates the trend of % seizure change over the timepoints comparing the normal free carnitine group with the low free carnitine group