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The tissues were counterstained with DAPI (4,6-diamidino-2-phenylindole) for 10 min at room temperature to label the nuclei and then mounted on slides with anti-fade medium (ProLong Gold, Thermo Fisher Scientific Inc., Rockford, IL)

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Conversely, in neuronal networks treated with d-lactate we observed an increase in apoptosis and cell death as compared to not treated cultures

The peptide operates through several identified mechanisms including upregulation of growth hormone receptors, activation of the FAK-paxillin pathway essential for cell migration, promotion of angiogenesis through VEGF modulation, and interaction with the nitric oxide system