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However, as with any supplement or treatment, there's potential for side effects, especially when B12 is given in very high doses

For systemic or gut-related protocols, abdominal subcutaneous injection is the standard approach

Standard assays of GST activity The activities of the GST stocks were assayed at 30 C with the standard substrates, 1 mM CDNB (stock solution in ethanol) and 1 mM GSH in 0.1 M sodium phosphate buffer pH 6.5, containing 1 mM EDTA, and the formation of the product S-2,4-dinitrophenyl-glutathione (DNP-SG) was followed spectrophotometrically at 340 nm 23

Prog Neuropsychopharmacol Biol Psychiatry 2008