It also protects liver cells from the oxidative damage that occurs during the filtration process
Despite these findings of higher total and unbound prednisolone concentrations, elderly subjects had higher AUCs of cortisol, suggesting that the elderly population is less sensitive to suppression of endogenous cortisol or their capacity for hepatic inactivation of cortisol is diminished
Continuing at room temperature, slides were rinsed three times with 1 PBS prior to the addition of secondary antibodies diluted in 2% NGS in 1 PBS and incubation at room temperature in the dark for 1 h
Acknowledgments We thank Gabriela Furlan, Noelia Maldonado and Alejandra Romero (Cell Culture Facility, Luciana Reyna (Molecular Biology Facility), Paula Abadie and Santiago Boccardo (Cytometry Facility), Pilar Crespo (Microscopy Facility), Victoria Blanco and Raul Villarreal (Animal Care Facility), Sergio Oms, Ariel Frontera, Diego Luti, Walter Requena, Cecilia Noriega, and German Romero (BIOPROAL - Animal Production Facility) for their skillful technical assistance at the CIBICI-CONICET/ Department of Clinical Biochemistry, School of Chemistry Sciences, National University of Cordoba
