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You want to avoid spraying directly onto the nasal septum (the cartilage dividing your nostrils), as this can cause irritation and is less effective for absorption

The degree of chromophore protonation is also dependent on the type of chromophore: while roGFP1 derived from the wild-type chromophore with the amino acid S65 in the chromophore displays a dominant excitation peak in the UV range, the roGFP2 variant derived from EGFP containing the S65T mutation has a main excitation peak at 490 nm (Hanson et al., 2004)

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