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The core peptide should still contain the same 15 residues in the same order

20 , 28 Even though cagrilintide activated a lesser number of neurons than sCT in the AP, NTS and LPBN, it resulted in similar metabolic effects as sCT on day 1 of injection suggesting that the metabolic effects cannot be predicted by quantitative activation

After five minutes, initiate the reaction by adding 20 L of the Cumene Hydroperoxide Solution into each well and mix thoroughly (it is recommended to use a multi-channel pipette)