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Select areas of the plates without inoculated as a control ( B ) and with PAO1 ( C ) show the PAO1 biofilm has a brighter yellow/green color

To probe the binding footprint, we quantified the frequency and character of base-specific interactions across all replicates, consistently revealing engagement with six base pairs from the minor groove (Supplementary Tables 59), which is in excellent agreement with our earlier FRET analysis with the F-DDH sequence