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Weight was tracked using a digital scale as an objective marker of inflammation

Gene expression of HSL, ATGL, CPT-1, ACC-1, FAT/CD36 and leptin was measured by the Taqman multiplex method using the ABI 7900 quantitative real-time RT-PCR instrument (Applied Biosystems, Rotkreuz, Switzerland) as described by Heim 50

8 weeks (5 vials): ~15 mL 2 bottles 16 weeks (10 vials): ~30 mL 3 bottles Alcohol Swabs One for the vial stopper + one for the injection site each day

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