Bacteriostatic water is less thermally sensitive than lyophilized peptides, but Canadian domestic sourcing still provides advantages: no customs delays, no international shipping variables, and consistent Canadian regulatory framework compliance
Each coverslip was washed three times with PBS, blocked with blocking solution (1x PBS, 10 % normal goat serum (NGS) and 0.3 % Triton X-100) for 30 min at RT and incubated with primary antibodies (anti-GFP (rabbit polyclonal, used at 1:1000 in IF, Abcam, Cat# ab6556, RRID:AB_305564), anti vGLUT1 (guinea pig polyclonal, used at 1:500 in IF, Synaptic Systems, Cat# 135 304, RRID:AB_887878)) diluted in blocking solution for 2 h at RT
For those with ME/CFS, autoimmune disorders, and degenerative neurological diseases like Parkinsons and Alzheimers, I advise aiming for no less than 500 pg/mL, with an optimal of at least 750 pg/mL
[Accessed on 4th November 2022] Useful Diagnostic Tests Nerve conduction test Complete blood count