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For ROS detection, peptide conjugate solution (400 M) and 10 M DCFH were mixed in a 1:1 (v/v) ratio, yielding a final peptide concentration of 200 M in a total volume of 100 l per well in 96-well plate

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Restoration of the NAD + levels by niacin, a NAD + precursor and B3 vitamin, provided health benefits, slowly reversing physical performance of some adult patients 15 .To find patients who have NAD + deficiency and could benefit from B3 treatment, we needed to set up validated technology and assess their values in the normal population

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