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The clarified cell lysate was either directly submitted to further protein purification steps or flash-frozen in liquid nitrogen after addition of 10% glycerol and stored at 80 C for use in biocatalytic reactions

Consequently, the composition of an individuals microbiota is shaped by factors such as age, sex, ethnicity, and geographical region, resulting in a uniquely personalized GI microbial ecosystem (212)

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Those details help with basic supplier checks, but researchers should still review current product records, policies, and support information before ordering