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The major obstacles to developing probes that can monitor GSH dynamics include the reaction reversibility and kinetics of the sensing reaction, as well as the high abundance of GSH (110 mM) inside cells

The N-terminal glycine amino group donates one nitrogen ligand to the copper, the imidazole nitrogen of the histidine side chain donates a second nitrogen ligand, and the deprotonated amide nitrogen of the Gly-His peptide bond donates a third nitrogen ligand

521 PURE might also enable studies on nonspecific decoding of codons through wobble-base pairing, which might highlight how new genetic codes might be used for more accurate ncAA incorporation

Ecotoxicol Environ Saf 173:273284 Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants