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This would explain how PKC might be involved in morphine-induced MOPr regulation, because there is little evidence to indicate that the G i / G o -coupled MOPr can couple directly to PKC activation

For intracellular cytokine, staining cells were fixed with BD Cytofix Cytoperm (BD Biosciences) for 20 min at 4 C, washed twice with 1x saponin buffer (0.5% (w/v) saponin [Carl Roth], 2 mM EDTA, 2% FCS, in PBS) and stained for IFN (see Table 1) overnight at 4 C in 1 saponin buffer

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Used needles and syringes should be placed in a FDA-cleared sharps disposal container (puncture-proof) rather than in household trash, to prevent needle-stick injuries