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FIGURE 1 2.1 Iron metabolism imbalance Iron, an essential trace element, primarily participates in metabolic processes in the forms of Fe 2+ and Fe 3+ (Lee and Roh, 2025)

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21 Statistical analysis All data were expressed as meanS.E.M

Mild global hypomethylation of BM-MSCs with 0.5 M 5-Aza-dC preserved stemness markers, enriched their secretome and, when either modified cells or conditioned medium were administered to cisplatin-induced premature ovarian failure mice, they outperformed unmodified counterparts in restoring AMH, E and FSH levels and in rebuilding healthy primordial-to-antral follicle populations [171]