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After 1 h, cells were washed five times for 2 min each on ice with cold buffer and blocked for 5 min with cold 20 mM glycine in cold buffer

To explore this notion, we installed the BDHI electrophile onto a set of chemical fragments (so called scout fragments) previously shown to drive covalent protein engagement with different electronic and steric properties 2 and assessed their proteomic reactivity

Unfortunately, among women who underwent surgery for all types of POP, 11% underwent reoperation 5 years later, and an additional 4% underwent reoperation 10 years later (Nssler et al