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MATERIALS AND METHODS Formalin-induced nociceptive behavioral test The time-effect curves of the control, morphine, and BPC-157 (from 10 to 40 g/kg) groups for the flinching response in the formalin test are shown in Figure 1

Promega) (for AMYRs) or coelenterazine h (Nanolight Technologies, Pinetop, AZ) (for CTR) was then added to cells at a final concentration of 1:1000 or 5 M, respectively, and incubated for a further 1015 min

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While cysteine-based approaches are more selective, the reaction is more complicated due to presence of multiple disulfide bonds in the antibody