previously published and described in detail by Actor et al
Ten micrograms of each protein sample were separated into a 10% SDS-PAGE gel and transferred onto a polyvinylidene fluoride membrane
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Apart from the uptake and distribution of HM, metabolomics, which is the study of the identification, quantification, and distribution of metabolites, such as sugars, alcohols, amino acids, organic acids, and polyamines, provides a better understanding of the plants responses to any interference, including HM stresses (Ghatak et al., 2018)