Acetone was added to the supernatant to precipitate proteins, which were subsequently resuspended in loading buffer as cytosolic proteins
For example, GSTF6 has been implicated in the synthesis of camalexin (Su et al., 2011), a phytoalexin that can accumulate strongly in cat2 in oxidative stress conditions (Chaouch et al., With the above caveats in mind, useful pointers can be obtained by combining information from biochemical competence and efficiency (Tables 24) with responsiveness at the transcript level (Figure 4)
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In brain, the properties of GSH transport in isolated rat brain mitochondria seemed to be different from those reported previously for kidney mitochondria, as they were influenced most by inhibitors of the tricarboxylate carrier, citrate, isocitrate, and benzenyl-1,2,3-tricarboxylate (Wadey et al., 2009)