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When endothelial dysfunction chokes off this supply, the mitochondria are forced to operate in a hypoxic environment, leading to inefficient energy production and a massive increase in the generation of mitochondrial reactive oxygen species (mtROS)

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While a variable number of Ahx spacers was inserted to minimize steric clash between the NLS and the MDM2-binding interface, the NLS containing stapled peptides showed comparable binding values for MDM2, and were found to have sub-micromolar affinity for importin in a direct fluorescence polarization (FP) assay