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The plasmid for the non-tagged SUN2 expression was cloned by cutting the SUN2-CFP plasmid 65 with EcoRI and BamHI, blunting with Phusion DNA Polymerase (New England Biolabs, M0530), and re-ligating the plasmid thus introducing a stop codon in frame with the SUN2 sequence

Ice crystals form within the solution and physically shear apart the peptide molecules, permanently damaging their structure

This microarray contains 304 antibodies against 147 phosphorylation sites of proteins involved in 16 signaling pathways

A: Once you achieve your desired weight, our medical team will work with you to develop a maintenance plan that may include tapering off the treatment while continuing to support your weight management through other means, such as diet and exercise