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Cell suspensions were passed through a 70 m and 40 m cell strainer (BD Bioscience), resuspended in 2.5 mL of 40% Percoll preparation (20 mL Percoll [GE Healthcare], 2.2 mL 10PBS, 27.8 mL HBSS without Ca 2+ and Mg 2+ [Sigma-Aldrich]) and layered onto 1.5 mL 60% Percoll (30 mL Percoll, 3.3 mL 10PBS, 16.7 mL HBSS)

doi: 10.1016/j.funbio.2015.05.001 45 FabbriS.SilvaC.HernandesE.OctavianoF.ThommazoA

Studies on its pathogenesis are mainly based on genetic and molecular pathological evidence, with abnormal aggregation and deposition of -synuclein (S) as the central pathological feature (225)

Despite an increase in urine calcium concentration, the relative saturation of calcium oxalate decreased due to citrate chelation of calcium and the concentration of undissociated uric acid decreased due to the significant increase in urine pH (23)