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baumannii , the chitosan derivative achieved an MIC of 32 g/mL, compared to higher MIC values of 128 g/mL for amikacin and tobramycin and 64 g/mL for tazobactam

2.15 Real-time quantitative PCR Total RNA was extracted from aortic tissue and endothelial cells using TRIzol reagent (Takara Bio Inc., 9108), following the manufacturers instructions