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Because it modulates cellular NAD+ availability and mitochondrial function, some protocols administer it in the morning to align peak NNMT inhibition with waking metabolic activity

NAD+/NADH ratio assays, SIRT1 activity assays, and mitochondrial respiration measurements (such as oxygen consumption rate via extracellular flux analysis) are standard methods for studying this combination's proposed effects in cell-based models, typically comparing single-compound and combined-compound treatment conditions against untreated controls

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Many users find that metabolic improvements persist for 2-4 weeks after discontinuing treatment, suggesting lasting changes in cellular function