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Mitochondrial imaging Immunofluorescence Imaging was performed on a STED-capable Stellaris DMI8 platform (Leica)

Genomic HA-tagged TBC1D9B HeLa Cells were generated with CRISPR/Cas9 mediated knockins using Alt-R sgRNA and ssODN Donors from IDT (Coralville, IA, USA) with the following sequences: Alt-R sgRNA: AGGCATCAGCCGGAAACTCC ssODN Donor: TGATGACACCTTGGGCCAGGCCTCACAGCTGCAGGCATCAGGCGTAGTCGGGGACGTCGTAGGGGTAGCCGGAAACTCCAGGCTGCTCATGGTCACTGGCGGTGCTGAACTGTCTC

Definition and diagnostic criteria Hereditary glutathione synthetase deficiency is a rare autosomal recessive disease characterized by hemolytic anemia, metabolic acidosis, 5-oxoprolinuria, progressive neurological symptoms and recurrent bacterial infections

It allows you to know that if a GH pulse is blunted, it's due to a factor like food timing, not a faulty product