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Similarly, the expression of key autophagy genes, such as ATG5 , ATG7 , and BECN1 , is downregulated during normal aging in the human brain [209]

The results revealed that the levels of pro-inflammatory cytokines IL-6, IL-12, and TNF- secreted by cells treated with NP4 were 1.45, 5, and 1.78 times higher than those secreted by cells treated with NP2, respectively, further confirming the activation of macrophages

This flexibility is particularly valuable for early-stage discovery work, where protocols evolve rapidly and the ability to fine-tune dose ranges is essential