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Briefly, cardiomyocytes were treated with PQQ as above and were then incubated for 20 min with a JC-1 working solution at 37 C, followed by two washed using JC-1 staining buffer

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61,62,63 The binding of PGD2 to the DP2 receptor induces proinflammatory downstream signaling pathways culminating in the activation and migration of Th2 cells and eosinophils to the inflammatory sites in asthma