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Electrospray ionisation mass spectrometry (ESI-MS) was used to identify a protonated molecular ion peak at m / z 308.2, which was found to be consistent with the expected mass

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FBCM is currently considered to be the most common cause of vitamin B12 deficiency, caused by impairment in the release of the vitamin from food, commonly because of conditions such as gastritis, achlorhydria, gastrectomy, use of antacids, or proton pump inhibitors (PPIs), gastric atrophy in the elderly, and Helicobacter pylori infection (63, 74)

This dose was chosen based on a previous dose-escalation study and subsequent work demonstrating its efficacy to drive weight loss in a short period of time 8