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After MTT incubation, the optical density (OD) value at 550 nm of each group was further recorded with a Multiskan GO spectrophotometer (Thermo Scientific, Beijing, China) ( Assessment of antioxidant enzyme activities and oxidative stress markers To assess antioxidant enzyme activities and oxidative stress markers, frozen-thawed spermatozoa of each group (repeated in triplicated) were collected and centrifuged at 800g for 20 min to separate spermatozoa from seminal plasma

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