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Administering a beta-blocker in this state eliminates vital compensatory cardiac mechanisms (such as increased contractility), leaving the -1-stimulation unopposed (Medical Advisory Board 2026b)

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For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C