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To assess the number of redox-sensitive Cys residues in GSAAT, the enzyme was treated with hydrogen peroxide or DTT (0.110 mM DTT) and subsequently with N-ethylmaleimide (NEM) to irreversibly block the free thiol groups of Cys residues in GSAAT

European Journal of Biomedical 8(12):408424 Elghareeb MM, Elshopakey GE, Rezk S, Ateya A, El-Ashry ES, Shukry M, Ghamry HI, Alotaibi BS, Hashem NM (2024) Nigella sativa oil restores hormonal levels, and endocrine signals among thyroid, ovarian, and uterine tissues of female Wistar rats following sodium fluoride toxicity

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